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Titlebook: Kidney Research; Experimental Protoco Tim D. Hewitson,Edward R. Smith,Stephen G. Holt Book 2016Latest edition Springer Science+Business Med

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书目名称Kidney Research
副标题Experimental Protoco
编辑Tim D. Hewitson,Edward R. Smith,Stephen G. Holt
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Kidney Research; Experimental Protoco Tim D. Hewitson,Edward R. Smith,Stephen G. Holt Book 2016Latest edition Springer Science+Business Med
描述.This second edition expands upon the previous volume with additional emphasis on recent innovation in basic renal research and has a more holistic approach on associated disorders such as complications associated with peritoneal dialysis, ischemic acute kidney injury, the sympathetic nervous system, and vascular calcification. The book is divided into five parts: Part I provides a number of .in vitro., .in vivo., and .ex vivo. models of kidney disease and associated complications; Part II looks at recent advances in imaging techniques; Part III covers recent developments in studying metabolism in renal ischemia and reperfusion; Part IV addresses study and measurement of vascular calcification; and Part V explores analytical techniques that are both topical and of widespread relevance to the study of experimental renal disease. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Kidney Research: Experimenta
出版日期Book 2016Latest edition
关键词renal anatomy; renal physiology; peritoneal dialysis; cardio-renal syndrome; vascular calcification; glom
版次2
doihttps://doi.org/10.1007/978-1-4939-3353-2
isbn_softcover978-1-4939-8028-4
isbn_ebook978-1-4939-3353-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
The information of publication is updating

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Propagation and Culture of Human Renal Fibroblastsf the fibroblast, its behavior, and factors affecting its activity is therefore key to understanding both its role and significance. In this protocol, we describe a reproducible method for selective propagation and culture of primary human renal fibroblasts from the human kidney cortex. Techniques f
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Isolation and Propagation of Rat Peritoneal Mesothelial Cellsis chapter we describe a reliable and reproducible method for the culture of rat primary mesothelial cells (RPMCs). This chapter outlines how to isolate mesothelial cells from rat peritoneum. The subculture of primary peritoneal mesothelial cells and the characterization by immunofluorescence is als
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Renal Sympathetic Denervation in Rats. While more recently, direct renal sympathetic denervation is typically applied by stripping all visible renal nerve bundles followed by painting with a solution of 10 % phenol in ethanol to remove the remaining nerves. In clinical settings, a reliable marker of renal sympathetic denervation or ren
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Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI morphological measurements can be made in intact organs and allow direct measurements of every perfused glomerulus. Cationic ferritin (CF) is used as a superparamagnetic contrast agent for MRI. CF binds to the glomerular basement membrane after intravenous injection, allowing direct, whole-kidney m
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