Titlebook: KRAS; Methods and Protocol Andrew G. Stephen,Dominic Esposito Book 2024 Leios Biomedical Research, Inc. 2024 GTPase activity.mass spectrome

善于

Affinity Measurement of Non-covalent Interactions of the Covalent KRAS G12C GDP Inhibitor MRTX849 tof molecular interactions but also association and dissociation rates of such interactions. In this chapter, we describe a general method to measure the affinity of a small molecule drug, MRTX849, to GDP bound HRAS, KRAS, and NRAS.

小鹿

perjury

Slit-Lamp

Nausea

KRAS4b:RAF-1 Homogenous Time-Resolved Fluorescence Resonance Energy Transfer Assay for Drug Discovees compared to traditional assay techniques. In this chapter, we describe an HTRF protein-protein interaction (PPI) assay for the KRAS4b G-domain in the GppNHp-bound state and the RAF-1-RBD currently used for drug screens. Application of this assay contributes to the identification of lead compounds targeting the GTP-bound active state of K-RAS.

Hiatal-Hernia

Amplify

978-1-0716-3824-8Leios Biomedical Research, Inc. 2024

MIME

KRAS978-1-0716-3822-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

模范

Andrew G. Stephen,Dominic EspositoIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

有斑点