Titlebook: Immunosenescence; Methods and Protocol Roberta Amoriello,Clara Ballerini,Alice Mariottini Book 2025 The Editor(s) (if applicable) and The A

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Method to Assess Immunosenescent CD8+ T Cells in Respiratory Viral Infections,scence, it is imperative to develop methods to accurately identify immunosenescent cells. Elderly patients are known to have impaired immune responses to respiratory viruses, and it is hypothesized that this is due, in part, to immunosenescent, terminally exhausted CD8. T cells. To test this hypothe

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Investigation of Signaling in Primary Populations of Human Senescent T Cells,tion cascades and are important for the activation, differentiation, and effector functions of lymphocytes. Recent studies on the aged immune systems from both humans and mice have uncovered a different mode of MAPK signaling that is independent of canonical activation cascades and instead occurs th

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Transcription Factor Analysis to Investigate Immunosenescence in Rheumatoid Arthritis Patients, ability of T cells. RA T cells develop a unique aggressive inflammatory senescent phenotype with an imbalance of Th17/T regulatory (Treg) cell homeostasis and presence of CD28. T cells. The phenotypic analysis and characterization of T cell subsets become necessary to ascertain if any functional de

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,Evaluation of Gene Expression Profiling by QuantiGene™ 2.0 RNA Assay,lar mechanism involved in the defects of the immune response during aging..QuantiGene™ 2.0 technique is a multiplex platform allowing the simultaneous analysis of several target RNA molecules (up to 80) present in a single sample. QuantiGene Assays use an accurate method for multiplexed or for singl

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Application of Multiplex Immunoassay in Aging Research: A Methodological Approach,ory state known as inflammaging. Although the progression of inflammaging is now recognized as one of the main driving forces of aging and one of the main risk factors for morbidity and mortality in older subjects, current knowledge on the causative agents of inflammaging itself and chronic, aging-r

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Analysis of Cytokine-Inducible SH2-Containing Protein (CISH) in the Study of Immunosenescence, immunosenescence is crucial for developing targeted interventions to enhance immune functions in older individuals. This bio-protocol review focuses on the application of quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for the mRNA quantification of cytokine-inducible SH2-con

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Computational Analysis of T-Cell Receptor Repertoire Workflow: From T-Cell Isolation to Bioinformat enormous diversity of the TCR repertoire, a crucial feature determining the individual capability to response to antigens and to build immunological memory. A pivotal role in the recognition of antigen is played by the hypervariable complementarity-determining region 3 (CDR3) of the V-beta chain of

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In Vitro Interaction Between Yeast Extracellular Vesicles and Human Monocyte-Derived Dendritic Cell such as nucleic acids and metabolites, and are involved in cell functioning, communication, and signaling. Recent literature reported that pathogenic or commensal yeast strains can produce EVs targeting the host’s immune system and exerting immunomodulatory actions. In humans, yeast EVs can be endo