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Titlebook: Iron Metabolism; Methods and Protocol Oleh Khalimonchuk Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license

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书目名称Iron Metabolism
副标题Methods and Protocol
编辑Oleh Khalimonchuk
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Iron Metabolism; Methods and Protocol Oleh Khalimonchuk Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license
描述.This detailed volume explores classical and cutting-edge methods optimized and validated to analyze various aspects of iron metabolism, from in vitro to multi-organ level. Opening with a section on basic iron metabolism methods, the book continues with methods applicable to a variety of systems, ranging from bacteria to cultured mammalian cells and tissues, with a focus on cellular heme and iron-sulfur cluster species, as well as mitochondrial iron and its derivatives. Written for the highly successful .Methods in Molecular Biology .series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls... ..Authoritative and practical, .Iron Metabolism: Methods and Protocols. serves as an ideal guide for researchers seeking to help in demystifying and boosting iron metabolism-related research..
出版日期Book 2024
关键词Micronutrient ; Bacteria; Mammalian cells and tissues; Intracellular iron; Ferrous; Ferric; Mitochondrial
版次1
doihttps://doi.org/10.1007/978-1-0716-4043-2
isbn_softcover978-1-0716-4045-6
isbn_ebook978-1-0716-4043-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Direct Spectroscopic Ferrochelatase Assaye coproheme III. Ferrochelatase activity in extracts or purified protein can be measured via several assays. Here, we describe a rapid real-time direct spectroscopic ferrochelatase assay for both protoporphyrin and coproporphyrin ferrochelatases.
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Methods in Molecular Biologyhttp://image.papertrans.cn/i/image/475392.jpg
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Iron Homeostatic Regulation in ,: Introduction to a Computational Modeling Methods and molecular-level regulatory mechanisms involved. Most advances have involved inferring such mechanisms based on the analysis of iron-dysregulation phenotypes arising in various genetic mutant strains. Still lacking is a cellular- or system-level understanding of iron homeostasis. These experime
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ICP-MS Analysis of Iron from Biological Samplesmmalian cells, subcellular compartments, biological fluids, and tissues. The best method of analysis is by far Inductively Coupled Plasma Mass Spectrometry (ICP-MS). I describe here a very general method for the sample preparation, instrument settings, method development, and analysis. The method ca
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