Titlebook: Ionotropic Glutamate Receptor Technologies; Gabriela K. Popescu Book 2016 Springer Science+Business Media New York 2016 atomic organizatio

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Assaying AMPA Receptor Oligomerizationnumber and composition of AMPARs on the plasma membrane impact synaptic strength, neurodevelopment, and brain disorders. While the mechanisms mediating oligomerization of AMPARs are not well understood, they form the template for defining the number and preferential assembly of AMPARs. In this chapt

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A Step-by-Step Guide to Single-Subunit Counting of Membrane-Bound Proteins in Mammalian Cellsst recurring issue is that composition and subunit stoichiometry is commonly inferred from bulk biochemical assays that can only shed light on the “averaged” makeup of the protein complex. However, recent studies have been able to circumvent this issue by studying the stoichiometry of . protein comp

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Electrophysiological Tagging of Ionotropic Glutamate Receptorsceptors. Study of the trafficking and synaptic incorporation of these receptors is critical to understanding the normal function and regulation of synapses as well as physiological processes like synaptic plasticity, and neuropathologies where glutamatergic synaptic transmission has been compromised

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Electron Microscopy Analysis of AMPA Receptors in Dendritic Spinesceptors present at each synapse are regulated physiologically and determine the strength of the synaptic response. High-resolution imaging techniques such as electron microscopy (EM) can inform about the ultrastructural localization of AMPA receptors in native tissue; in addition, immunogold stainin

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