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Titlebook: Investigations of Cellular and Molecular Biophysical Properties by Atomic Force Microscopy Nanorobot; Mi Li Book 2018 Springer Nature Sing

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书目名称Investigations of Cellular and Molecular Biophysical Properties by Atomic Force Microscopy Nanorobot
编辑Mi Li
视频video
概述Awarded as an Outstanding PhD thesis by the Chinese Academy of Sciences in 2015.Describes a comprehensive study on using atomic force microscopy (AFM) to investigate single-cell and single-molecule ac
丛书名称Springer Theses
图书封面Titlebook: Investigations of Cellular and Molecular Biophysical Properties by Atomic Force Microscopy Nanorobot;  Mi Li Book 2018 Springer Nature Sing
描述.This book presents methodological and application research in detecting cellular and molecular biophysical properties based on atomic force microscopy (AFM) nanorobotics. Series methods for in situ label-free visualizing and quantifying the multiple physical properties of single cells and single molecules were developed, including immobilization strategies for observing fine structures of living cells, measurements of single-cell mechanics, force recognition of molecular interactions, and mapping protein organizations on cell surface. The biomedical applications of these methods in clinical lymphoma treatments were explored in detail, including primary sample preparation, cancer cell recognition, AFM detection and data analysis. Future directions about the biomedical applications of AFM are also given..
出版日期Book 2018
关键词Atomic Force Microscopy; AFM; Lymphoma targeted therapy; Cell mechanics; Single-molecule force spectrosc
版次1
doihttps://doi.org/10.1007/978-981-10-6829-4
isbn_softcover978-981-13-4954-6
isbn_ebook978-981-10-6829-4Series ISSN 2190-5053 Series E-ISSN 2190-5061
issn_series 2190-5053
copyrightSpringer Nature Singapore Pte Ltd. 2018
The information of publication is updating

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2190-5053 copy (AFM) to investigate single-cell and single-molecule ac.This book presents methodological and application research in detecting cellular and molecular biophysical properties based on atomic force microscopy (AFM) nanorobotics. Series methods for in situ label-free visualizing and quantifying th
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Single-Molecule Recognition and Force Measurements by AFM,, the CD20-rituximab specific interactions were probed on purified CD20s coated on mica and on native CD20s on the surface lymphoma Raji cell, showing the different molecular binding forces of purified CD20s and native CD20s.
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Immobilization Methods for Observing Living Mammalian Suspended Cells by AFM,d combining lateral micro-pillar/micro-well mechanical trapping and vertical poly-L-lysine electrostatic adsorption was developed, with which single living human lymphoma cells were effectively trapped for AFM imaging with high quality, facilitating further AFM morphological studies of living mammalian suspended cells.
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