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Titlebook: Invariant Natural Killer T-Cells; Methods and Protocol Chaohong Liu Book 2021 The Editor(s) (if applicable) and The Author(s), under exclus

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书目名称Invariant Natural Killer T-Cells
副标题Methods and Protocol
编辑Chaohong Liu
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Invariant Natural Killer T-Cells; Methods and Protocol Chaohong Liu Book 2021 The Editor(s) (if applicable) and The Author(s), under exclus
描述This detailed book focuses on various aspects of Invariant Natural Killer T-Cells (iNKT), which are known to contribute to homeostasis and autoimmunity and can also cause various pathological responses such as allergy, infection, excessive autoimmune response, and cancer. The volume explores techniques for identification and isolation of iNKT cells, iNKT cell activation and transformation, as well as proliferation and differentiation and much more. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Invariant Natural Killer T-Cells: Methods and Protocols. is an ideal guide for researchers working with and studying iNKT cells, perhaps to provide a deeper knowledge of the human immune system..
出版日期Book 2021
关键词Cell identification; Cytokines; Cell activation; iNKT cells; Pathological responses; Immune response
版次1
doihttps://doi.org/10.1007/978-1-0716-1775-5
isbn_softcover978-1-0716-1777-9
isbn_ebook978-1-0716-1775-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Detection of Mouse Type I NKT (iNKT) Cells by Flow Cytometry,iplier tubes or photodiodes for detection. Flow cytometry allows immunophenotyping using fluorescently conjugated antibodies for the identification of subgroups of immune cells at a single-cell level. Natural killer T (NKT) cells are CD1d-restricted T cells, which recognize lipid antigens, unlike co
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Identification of Rare Thymic NKT Cell Precursors by Multiparameter Flow Cytometry,une responses. Features of innate responses are attributed to these cells because they can be stimulated simultaneously with the same ligand to produce quickly and in large amount cytokines without prior immunization. Because these characteristics could be exploited for clinical applications, NKT ce
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Expansion of Human iNKT Cells Ex Vivo,ells ex vivo is essential for their translational usage. The culturing procedure described here provides an optimized method for ex vivo expansion of iNKT cells using recombinant human IL-15 (rhIL-15) and recombinant human IL-12 (rhIL-12), which results in cell products with enhanced cytokine secret
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