Titlebook: Introduction to Fluorescence Sensing; Volume 2: Target Rec Alexander P. Demchenko Book 2023Latest edition Springer Nature Switzerland AG 20

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Fluorescence Sensing of Physical Parameters and Chemical Composition in Gases and Condensed Media,al conditions involves obtaining quantitative fluorescence response to temperature, pressure, polarity, viscosity, and the rates of dielectric relaxation and of molecular diffusion. Structural transformations in these media can be probed on molecular level. Different analytes can be detected in a mo

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Quantitative Fluorescent Detection of Ions,ists regarding selection and operation of recognition units. Meantime, three major principles in fluorescence reporting dominate in ion sensing. They are the ionization behavior of coupled to fluorophore charged groups, the modulation of photophysical processes in fluorophore (such as electron, prot

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Detection and Imaging of Small Molecules of Biological Significance, in the present chapter. Due to small strength and number of potential contacts with the sensor, molecular recognition may not be very efficient, and their detection can be based on chemodosimetry concept. Many of these compounds possess specific reactivity that is the basis of their function, and t

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Detection, Structure and Polymorphism of Nucleic Acids,nsic detection. Such extremely broad range of applications requires the search of diverse methodologies that should be optimal for every particular task. Fluorescence techniques offer a broad range of possibilities, from quantitative nucleic acid detection and visualization in cells to characterizin

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Fluorescence Detection of Peptides, Proteins, Glycans,stics. Their largely variable molecular weights, complex structures and high functional diversity create great problems for their specific detection. In this chapter we analyze these problems and describe their solutions. Two major trends can be outlined—detection of individual species with highest

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Detection of Harmful Microbes,diseases. Strong move from culture-cultivation methods to direct sensing that could avoid enrichment and purification processes is a goal that must be reached in near future. Fluorescence-based sensing methods are highly attractive in this respect owing to their real-time, economic, highly selective

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Fluorescent Imaging In Vivo,dy. Will this sensing have a prospect in application to humans addressing the needs of clinical diagnostics and treatment? What are the technological challenges here and the possibilities for their solution? Here we address the problems related to the technologies of introducing the imaging agents a