Titlebook: Introduction to Enzyme Technology; Karl-Erich Jaeger,Andreas Liese,Christoph Syldatk Textbook 2024 The Editor(s) (if applicable) and The A

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Enzyme Identification and Screening: Activity-Based Methodsnt years. The use of activity-based methods for enzyme identification from natural resources such as the metagenome allows the discovery of biocatalysts. The main advantage of activity-based screening is the direct identification of desired enzyme activities completely independent of any knowledge a

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Bioinformatic Methods for Enzyme Identificationining the sequence identity of two protein or DNA molecules, finding homologous sequences of a known enzyme, or analyzing complex relationships of whole organisms. This chapter describes different bioinformatic methods and respective tools that will enable you to search for new protein sequences, wh

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Optimization of Enzymesmain concepts—rational protein design and directed evolution—are introduced and the major differences are explained. Molecular biology methods for generating mutant libraries by site-directed mutagenesis or random mutagenesis techniques are described, as well as concepts for screening or selection t

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Principles of Applied Biocatalysisself, the reaction control of biocatalytic reactions is an essential part in the preparative synthesis of important products or intermediates. Thus, for the efficient performance of the planned biocatalytic processes, the understanding of the limiting factors and the use of appropriate strategies to

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