Titlebook: Introduction to Cell and Tissue Culture; Theory and Technique Jennie P. Mather,Penelope E. Roberts Book 1998 Springer Science+Business Medi

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Serum-Free Culture,cultures, which are covered in Chapter 5. Several elements of the role of serum in cell growth media and the substitutes used in serum-free culture are summarized. (See Table 4.3 for commercially available media specifically designed for serum-free culture.)

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Cell Culture for Commercial Settings,ly to cell culture as performed in commercial settings. As with previous chapters on specialized tissue culture techniques, we will not attempt to give complete details on techniques used in this setting, but will refer to published material describing techniques in each subdiscipline.

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Setting Up a Cell Culture Laboratory,he introduction of potential contaminants. Traffic in and out of the culture room (space) and talking in the space are to be discouraged. All tasks that do not need to be performed in the culture room (e.g., which do not require a sterile environment) should be performed elsewhere. People not active

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Media, or another complex biological fluid (milk, embryo extracts, and plasma are examples), or, as discussed in Chapter 8, with a defined mixture of hormones and growth factors. The ongoing experimental work of replacing the complex mixtures with defined components, both nutrients and proteins, largely h

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Standard Cell Culture Techniques,ese few techniques, he or she will be able to perform routine maintenance of cell lines and set up cells for most biochemical or cell culture experiments. The more complicated techniques needed for special types of culture, the special considerations to be kept in mind when doing serum-free culture,

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