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Titlebook: Intracellular Lipid Transport; Methods and Protocol Guillaume Drin Book 2019 Springer Science+Business Media, LLC, part of Springer Nature

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发表于 2025-3-21 18:58:05 | 显示全部楼层 |阅读模式
书目名称Intracellular Lipid Transport
副标题Methods and Protocol
编辑Guillaume Drin
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Intracellular Lipid Transport; Methods and Protocol Guillaume Drin Book 2019 Springer Science+Business Media, LLC, part of Springer Nature
描述.This detailed book provides technical approaches to tackle a variety of questions related to intracellular lipid transport in order to improve our understanding at different scales of how lipids are accurately displaced between organelles, across long distances or at membrane contact sites, or within cellular membranes. The volume begins with methodologies to measure the movement of varied lipid species between or in organelle membranes, inside eukaryotic cells, including plant cells, or in bacteria, and continues in vitro or in silico approaches aiming to define, more from a biochemical and structural standpoints, how lipid transfer proteins (LTPs) or flippases/scramblases precisely function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. Authoritative and practical, .Intracellular Lipid Transport: Methods and Protocols. serves as an ideal guide for researchers seeking to shed light on diverse aspects of this critical and often elusive cel
出版日期Book 2019
关键词Lipid transfer proteins (LTPs); Membrane contact sites; Sphingolipids; Glycerophospholipids; Organelle m
版次1
doihttps://doi.org/10.1007/978-1-4939-9136-5
isbn_ebook978-1-4939-9136-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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发表于 2025-3-21 20:18:56 | 显示全部楼层
Intracellular Lipid Transport978-1-4939-9136-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Indirect Lipid Transfer Protein Activity Measurements Using Quantification of Glycosphingolipid Proe yet that directly would address the lipid transfer protein activity in vivo. Therefore, these approaches can serve as tools to indirectly study the lipid transfer protein activity in cells, by monitoring their impact on the glycosphingolipid homeostasis.
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1064-3745 experts.This detailed book provides technical approaches to tackle a variety of questions related to intracellular lipid transport in order to improve our understanding at different scales of how lipids are accurately displaced between organelles, across long distances or at membrane contact sites,
发表于 2025-3-23 03:07:02 | 显示全部楼层
Bi- and Trifunctional Lipids for Visualization of Sphingolipid Dynamics within the Cell,l sphingosine to reveal its subcellular localization via crosslinking, fixation, and specific staining by click reaction with a fluorophore. Additionally, these probes allow investigations into lipid metabolism as revealed by thin-layer chromatography.
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Advanced In Vitro Assay System to Measure Phosphatidylserine and Phosphatidylethanolamine Transporte to assess not only the ER-to-mitochondria but also mitochondria-to-ER transports at the same time. We believe that this assay system can accelerate the research on inter-organelle phospholipid trafficking.
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