Titlebook: Innate Immune Activation; Methods and Protocol Dominic De Nardo,Christine M De Nardo Book 2018 Springer Science+Business Media LLC 2018 NFB

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Biochemical Isolation of the Myddosome from Murine Macrophages,pathways essential for host defense. Despite their structural divergence, key signaling molecules in diverse innate immune pathways mediate signal transduction by assembling higher-order protein complexes at specific subcellular locations in a stimulus-dependent manner. These protein complexes are c

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Inflammatory Caspases: Activation and Cleavage of Gasdermin-D In Vitro and During Pyroptosis,l inflammasome activation or caspase-4, 5, and 11 upon their ligation and activation by cytosolic LPS. Upon a single cleavage between the two domains in Gasdermin-D, the N-terminal domain binds to membrane lipids and lyses cells by forming pores of an inner diameter of 10–14 nm within the membrane.

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Detection of ASC Speck Formation by Flow Cytometry and Chemical Cross-linking,flammasomes. This unique feature of ASC allows its visualization by several imaging techniques and constitutes a reliable and feasible readout for inflammasome activation in cells and tissues. We have previously described step-by-step protocols to generate immortalized cell lines stably expressing A

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