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Titlebook: In Vivo Fluorescence Imaging; Methods and Protocol Mingfeng Bai Book 2016 Springer Science+Business Media New York 2016 Fluorescence imagin

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Noninvasive Imaging of Fluorescent Reporters in Small Rodent Models Using Fluorescence Molecular Toight passing through tissues mean that the choice of reporters, models, and imaging systems needs to be carefully considered. Here, we describe approaches to design and run experiments incorporating noninvasive whole animal fluorescence imaging into small animal imaging studies.
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Small-Animal Imaging Using Diffuse Fluorescence Tomography,. However, deploying DFT effectively is complex and often requires years of know-how, especially for newer mutlimodal systems that combine DFT with conventional imaging systems. In this chapter, we step through the process of using MRI-DFT imaging of a receptor-targeted tracer in small animals.
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In Vivo Metal Ion Imaging Using Fluorescent Sensors,sion and can inform the development and translation of therapeutics. Herein, we present theories behind small-molecule, fluorescent, metal ion sensors as well as the methods for their successful application to in vivo metal ion imaging, including ex vivo validation.
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Using Fluorescence Imaging to Track Drug Delivery and Guide Treatment Planning In Vivo, assessment and treatment planning of siRNA delivery into the orthotopic tumor, thus enabling efficacious RNA interference (RNAi) therapy. In this chapter, we introduce this method to illustrate the use of CT-FMT co-registration for tracking drug delivery and guiding treatment planning in vivo.
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Experimental Design and Data Analysis of In Vivo Fluorescence Imaging Studies,second half of this chapter, we explain the fundamental statistical concepts and methods used in the data analysis of typical in vivo experiments. We also provide specific examples in in vivo imaging studies to illustrate the key steps of analysis procedure.
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In Vivo Fluorescence Reflectance Imaging with Subcutaneous Mouse Tumor Models,cations, fluorophores with long emission at the near-infrared (NIR) region are generally preferred to overcome the photon attenuation in living tissue. Here, we describe the in vivo fluorescence imaging of an integrin α.β. targeted NIR fluorescent probe (cRGD-ICG-Der-02) using subcutaneous mouse tumor models.
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