Titlebook: In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs; Shinichi Ohno,Nobuhiko Ohno,Nobuo Ter

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Book 2016 real in vivo morphology and immunolocalization of the molecular components in living animal organs. Recently, the live imaging of cells and tissues of animals with fluorescence-labeled proteins by gene manipulation has become more and more popular in biological fields. Current research, meanwhile,

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How to Perform “IVCT”simultaneously cryocut with a precooled cryoknife in liquid nitrogen (−196 °C) with a group of three persons. The in vivo frozen animal organs were cracked off from the animal body in the liquid nitrogen and then plunged into the liquid nitrogen. To perform the IVCT more easily, the “in vivo cryoapp

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