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Titlebook: In Vitro Neurochemical Techniques; Alan A. Boulton,Glen B. Baker,Alan N. Bateson Book 1999 Humana Press 1999

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书目名称In Vitro Neurochemical Techniques
编辑Alan A. Boulton,Glen B. Baker,Alan N. Bateson
视频video
丛书名称Neuromethods
图书封面Titlebook: In Vitro Neurochemical Techniques;  Alan A. Boulton,Glen B. Baker,Alan N. Bateson Book 1999 Humana Press 1999
描述In Vitro Neurochemical Techniques is the third work updating and expanding the best-selling inaugural volume of Humana Press‘s warmly received Neuromethods series, General Neurochemical Techniques (vol. 1). The key techniques detailed in this new edition encompass the breadth of neurochemical and molecular neurobiology research, ranging from the isolation of neuronal genes and the study of their expression to the analysis of receptor-ligand interactions, to the characterization of the consequences of receptor activation. The methods include electrophysiological techniques to explore the functional properties of receptors present in the membranes of excitable cells, methods to isolate novel genes central to neurobiological processes, and protocols to perform in situ hybridization histochemistry. Other methods cover the measurement of changes in gene expression, the rapid identification of gene polymorphisms, and the identification and characterization of second messenger pathways..The companion volumes, In Vivo Neuromethods and Cell Neurobiology Techniques, cover both in vivo methods and in vitro cell neurobiology approaches. Like the original, all three cutting-edge works will prov
出版日期Book 1999
版次1
doihttps://doi.org/10.1385/0896035093
isbn_softcover978-1-61737-055-7
isbn_ebook978-1-59259-639-3Series ISSN 0893-2336 Series E-ISSN 1940-6045
issn_series 0893-2336
copyrightHumana Press 1999
The information of publication is updating

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Electrophysiological Studies on Receptors In Vitro,ranes, yet physiologically distributed according to their functional roles. In this Chapter, we will survey in an eclectic manner, a number of contemporary electrophysiological recording techniques commonly used to characterize “chemical” forms of membrane excitability expressed by different cell ty
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A Novel PCR-RFLP Detection Method Using an Optimized Set of Restriction Enzymes,r simplicity and speed of genotyping large numbers of individuals are represented by oligodeoxynucleotide hybridization assay, the oligonucleotide ligation assay, allele-specific PCR amplification or, if the DNA variation is detected by a restriction enzyme (RE), PCR-restriction fragment length poly
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Western Blot Analysis,e preparation of cell extracts, and the choice of antibodies, followed by a detailed description of the most common and reliable procedures that we have adapted and use routinely and satisfactorily. The reader wishing greater detail about alternate protocols is directed to other available sources (H
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