书目名称 | In Vitro Mutagenesis Protocols | 副标题 | Third Edition | 编辑 | Jeff Braman | 视频video | | 概述 | Establishes protein structure, function, activity, and interrelationships by authors of peer reviewed articles published in respected journals.Covers computational methods to evaluate protein stabilit | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of .In Vitro Mutagenesis Protocols. represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful .Methods in Molecular Biology.™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls...Authoritative and up-to-date, .In Vitro Mutagenesis Protocols, Third Edition. offers today‘s | 出版日期 | Book 2010Latest edition | 关键词 | BAC; Chromosom; DNA; Escherichia coli; Microbial systems; Nucleotide sequences; PCR; Protein regulation; Pro | 版次 | 3 | doi | https://doi.org/10.1007/978-1-60761-652-8 | isbn_softcover | 978-1-4939-5666-1 | isbn_ebook | 978-1-60761-652-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC 2010 |
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