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Titlebook: In Vitro Culture of Higher Plants; R. L. M. Pierik Book 1997 Springer Science+Business Media Dordrecht 1997 Embryo.development.fertilizati

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楼主: 管玄乐团
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978-0-7923-4527-5Springer Science+Business Media Dordrecht 1997
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Preparation and composition of nutrient media,The in vitro growth and development of a plant is determined by a number of complex factors:
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Sterilization of plant material,In principle there are 4 sources of infections: the plant (internal as well external), the nutrient medium (insufficiently sterilized), the air, and the research worker (inaccurate work). The most important of these is the plant itself, and plant material should be well sterilized before being isolated in vitro.
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Mechanization,If machines are used for in vitro culture, then it is certain that liquid media are being used. This has the following consequences (Bonga and Durzan, 1982):
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