书目名称 | In Situ Hybridization Methods |
编辑 | Giselbert Hauptmann |
视频video | http://file.papertrans.cn/463/462986/462986.mp4 |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
丛书名称 | Neuromethods |
图书封面 |  |
描述 | .This volume. .contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular .Neuromethods. series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory..Essential and authoritative, .In Situ Hybridization Methods .provides detailed protocols for newcomers to ISH, and inspires rese |
出版日期 | Book 2015 |
关键词 | TransISH; chromogenic RNA ISH techniques; fluorescent RNA ISH techniques; gene expression; genetic model |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-2303-8 |
isbn_softcover | 978-1-4939-4463-7 |
isbn_ebook | 978-1-4939-2303-8Series ISSN 0893-2336 Series E-ISSN 1940-6045 |
issn_series | 0893-2336 |
copyright | Springer Science+Business Media New York 2015 |