Titlebook: Immunosenescence; Methods and Protocol Albert C. Shaw Book 2015 Springer Science+Business Media 2015 adaptive immune responses.autophagy.ch

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Book 2015cluding approaches to the analysis of antigen receptor repertoire. In addition, .Immunosenecence: Methods and Protocols .guides readers through cell biology and biochemical methods for analyses of telomere dysfunction, autophagy, protein oxidation, microRNAs and DNA methylation. Written in the highl

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Monitoring the DNA Damage Response at Dysfunctional Telomeres, an opportunity to quantify the extent of telomere dysfunction and monitor downstream DNA damage signaling pathways. Here we describe a method for the detection of TIFs using a fluorescent in situ hybridization (FISH) approach.

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,Single-Cell Analysis of T-Cell Receptor αβ Repertoire,els of TCRα and TCRβ-specific primers in a nested PCR to amplify expressed transcripts from individual, epitope-specific T cells. This strategy yields the paired TCRαβ sequences of any given population of αβ T cells of interest.

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Laboratory and Data Analysis Methods for Characterization of Human B Cell Repertoires by High-Throu much greater insight into clinically relevant B cell responses and memory storage. Here, we describe strategies for preparing and analyzing human antibody gene libraries for studying B cell repertoires.

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Book 2015 reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Immunosenecence: Methods and Protocols .provides a mixture of basic and advanced protocols that will be useful for immunologists in general and investigators in aging biology in particular..

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Isolation of Lipid Rafts from Human Neutrophils by Density Gradient Centrifugation,uman neutrophils. Cells are lysed in an HEPES solution containing 0.5 % Triton X-100, supernatants are mixed with a 42 % sucrose solution, which is then overlaid with a 35 % and 5 % sucrose solution. The gradient is centrifuged for 16 h and the resulting fractions can be further analyzed by immunoblotting or subjected to immunoprecipitation.

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Analysis of DNA Methylation by Pyrosequencing,ern of DNA has the potential to serve as an early detection marker and potential drug target for several diseases. Here, we provide a detailed technical protocol for pyrosequencing supplemented by critical information about assay design and nuances of the system that provides a strong foundation for beginners in the field.

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1064-3745 ation advice from the experts.In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire. In addition, .Immunosenecence: Methods and Protocols .