Titlebook: Immunometabolism; Methods and Protocol Suresh Mishra Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Innate im

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Immunometabolism and Its Potential to Improve the Current Limitations of Immunotherapy, have shown encouraging results across a multitude of malignancies and have shifted the paradigm of cancer treatment. However, no more than 40% of patients treated with these immune checkpoint blockade inhibitors respond. Thus, resistance is a barrier to therapy that remains poorly understood. All c

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Isolation and Preparation of Bone Marrow-Derived Immune Cells for Metabolic Analysis,e bone marrow may be subjected to metabolic assays for analysis or used to test the effect of infectious agents on immune cells. Here, we describe a process for the isolation of macrophages, dendritic cells, and neutrophils from mice. Using the methods described herein, specific immune cells with pu

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Methods in Molecular Biologyhttp://image.papertrans.cn/i/image/462255.jpg

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978-1-0716-0804-3Springer Science+Business Media, LLC, part of Springer Nature 2020

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Analysis of Immune-Tumor Cell Interactions Using a 3D Co-culture Model,l-cell interactions in the appropriate spatial dimensions. Here I describe the isolation and co-culture of immune cells with tumor cell lines in a three-dimensional system facilitated by a basement membrane extract. This allows for further downstream applications to analyze interactions between these cell types.

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https://doi.org/10.1007/978-1-0716-0802-9Innate immune system; Macrophages; Adipose and immune cells; Therapeutic targets; Metabolic pathways

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Book 2020and tips on troubleshooting and avoiding known pitfalls. .Authoritativeand practical, .Immunometabolism: Methods and Protocols. serves as a vital guide for researchers working at the important interface of immunology and metabolism..