Titlebook: Immunological Tolerance; Methods and Protocol Ashleigh S. Boyd Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019

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Book 2019ecessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .Immunological Tolerance: Methods and Protocols. aims to ensure successful results in the further study of this vital field..

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Isolation of Human Regulatory T Lymphocytes by Fluorescence-Activated Cell Sorting regulatory T cells, with high yield and purity, from immunostained PBMCs. Prior to FACS isolation, this protocol exploits magnetic immunoselection for pre-enrichment of CD25. PBMC, which reduces the duration of the subsequent FACS isolation.

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Immunological Tolerance978-1-4939-8938-6Series ISSN 1064-3745 Series E-ISSN 1940-6029

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