Titlebook: Immunological Techniques in Insect Biology; Lawrence I. Gilbert,Thomas A. Miller Book 1988 Springer-Verlag New York Inc. 1988 amphibians.b

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ELISA and Monoclonal Antibodies,tion” enables researchers to selectively monitor molecules of interest among a mixture of potentially interfering materials found in complex biological samples. Classical immunochemical techniques such as immunodiffusion and immunoprecipitation (see Chapter 1) do not in some cases offer sufficient d

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Time-Resolved Fluorescence Immunoassay,and sensitive binding of antigens to antibodies. Originally, radioisotopes were used for labeling the hormone or the antibody to measure the hormone titers via radioimmunoassay (RIA) or immunoradiometric assay (IRMA). Although this approach can still be of great value, applications of radioisotopes

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Immunocytochemistry,in tissues and cells. The techniques are basically rather simple. As most of the necessary reagents are commercially available, every laboratory is now able to carry out standard ICC experiments, provided the specific antibodies (ABs) are (made) available to recognize the Ag of interest. This chapte

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Immunoassay of Pesticides,rices. However, until recently their application has been largely limited to the clinical chemistry, endocrinology, and biochemistry laboratory. Acceptance of the techniques outside these areas has been slow, but interest is finally growing in their application to other analytical problems such as p

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Radioimmunoassay: Ecdysteroids,lated steroids—the ecdysteroids. The control of the hemolymph ecdysteroid titer is an extremely complicated process, being a result of both synthetic and degradative events, with regulation at the level of the prothoracic glands being primary. In response to developmental or environmental events, a

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Radioimmunoassays and ELISAs: Peptides,thodological advance in endocrinology in the past 50 years. The ability to measure notoriously small amounts of hormone in blood samples has decisively shaped our current concepts regarding endocrine mechanisms, especially in vertebrates. The impact of this technique on invertebrate endocrinology is

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