Titlebook: Immunochemical Protocols; Robert Burns Book 2005Latest edition Humana Press 2005 PCR.cell lines.chemistry.electron microscopy.microscopy

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Conjugation of Antibodies to Fluorescein or Rhodamine,The isothiocyanate derivative of the fluorophore, fluorescein or rhodamine, is coupled to the amino groups of IgG antibody in a one-step procedure and excess label is removed by gel filtration.

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Conjugation of Antibodies to Biotin,Antibodies can be conjugated to biotin by a number of chemical means. They can then be used in immunochemical procedures in conjunction with secondary reagents coupled to biotin-binding protein proteins such as avidin.

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incredulity

Dual-Antibody Sandwich Enzyme-Linked Immunosorbent Assay,Antibodies can be used to measures levels of molecules in solution using the dual-antibody sandwich enzyme-linked immunosorbent assay. Two antibodies are used that become bridged in the presence of the substance of interest, leading to the production of a colored product by a reporter enzyme.

Confound

Competitive Enzyme-Linked Immunosorbent Assay,Competitive enzyme-linked immunosorbent assay provides an alternative to dual-antibody sandwich enzyme-linked immunosorbent assay and is widely used for the measurement of substances in biological liquids.

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Epitope Mapping,Epitope mapping can be used to identify areas of a protein that an antibody recognizes and binds to. Monoclonal antibodies are easier to characterize, but epitope maps can also be produced for polyclonal antisera.

Instrumental

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Conjugation of Antibodies to Horseradish Peroxidase,eriodate to produce aldehyde groups. Then, in the second step, the aldehyde groups are allowed to react with amino groups in the IgG antibody. The Schiff bases formed are reduced and the conjugate is purified by gel filtration.

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Robert Burnshe real world..Probabilities defined on certain classes of sets of this universe, representing different states of knowledge about the world, have the properties of the major functions of the Dempster-Shafer calculus of evidence: belief functions and mass assignments. The importance of these epistem