Titlebook: Immunity in Insects; Federica Sandrelli,Gianluca Tettamanti Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 h

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书目名称Immunity in Insects影响因子(影响力)




书目名称Immunity in Insects影响因子(影响力)学科排名




书目名称Immunity in Insects网络公开度




书目名称Immunity in Insects网络公开度学科排名




书目名称Immunity in Insects被引频次




书目名称Immunity in Insects被引频次学科排名




书目名称Immunity in Insects年度引用




书目名称Immunity in Insects年度引用学科排名




书目名称Immunity in Insects读者反馈




书目名称Immunity in Insects读者反馈学科排名

Champion

Basic Methods to Evaluate Humoral Immunity Processes in Lepidoptera Larvaescrepancies between them often exist. Such discrepancies are mostly due to the different biology of animal models and to the nature of experimental approach. Here we described less time-consuming and cheaper protocols used to test the activity of both constituent and inducible humoral components present in the hemolymph of insect larvae.

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Preparation of Insect Protein Samples for Label-Free Proteomic Quantification by LC-Mass Spectrometrirections to further our understanding of protein function and their networks. This is particularly true for the characterization of the insect immune system. The aim of this work is to provide a comprehensive methodology to prepare insect samples for proteomic analysis.

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Quantification of Blood Cells in , and Other Insectstification. In addition, we summarize a method for the quantification of crystal cells, based on melanization. We discuss adaptations of the protocols for other blood cell types and other insect species and extend them for the combined use with cell biological approaches.

追踪

Detection of Enzyme Distribution, Expression, Activation, and Activity of Insect Prophenoloxidasen in insect tissues and cells, prokaryotic expression of PPO, PPO activation, and enzyme activity. The use of these methods may be helpful to scientists who are engaged in insect PPO and melanization research.

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Methods for the In Vitro Examination of the Antibacterial and Cytotoxic Activities of Antimicrobial ctericidal concentration are reported in detail. For the evaluation of the possible cytotoxic effect toward mammalian cells, the hemolytic test and the colorimetric assay based on the reduction of 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide are also described.

Fatten

Book 2020anscriptomic approaches, insect samples for proteomic analysis, hemocytes in .Drosophila, .cellular response in .Lepidoptera, .insect AMPs, manipulate the composition of mosquito microbiota, viral infections in insects, infections by entomopathogenic nematodes, immune response following oral infecti

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Discovery and Analysis of RNA Viruses in Insectsct-virus interactions, coevolution, and insect immune defenses. We describe here a bioinformatic pipeline for the discovery of RNA viruses from insects based on RNA sequence data and the analysis of insect antiviral immune response against the discovered viruses by using small RNA sequence data.