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Titlebook: Immobilised Macromolecules: Application Potentials; U. B. Sleytr,P. Messner,M. Sára Book 1993 Springer-Verlag London 1993 Biosensors.Immob

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S. Birnbauml PCR (ddPCR) is rapidly taking over for the more popular qPCR. In this chapter, we describe the detailed procedure based on droplets partitioning to quantify both linear and circRNA abundancy and demonstrate the circularity of the transcript under study with high precision, in a single experiment.
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P. W. Feldhoffontaining buffer (rather than K.) to destabilize G-quadruplexes. This biochemical method enables higher enrichment (two- to threefold) of circRNAs to be obtained compared to standard RNase R protocols due to more efficient removal of linear RNAs. By then performing quantitative RT-PCR (RT-qPCR) or g
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M. Sára,S. Küpcü,C. Weiner,S. Weigert,U. B. Sleytral assessment of small- and medium-sized circRNAs. Employing human-induced pluripotent stem-cell-derived cardiomyocytes originating from healthy controls or patients suffering from hypertrophic cardiomyopathy, we identify deregulated circRNAs and alternative exon usage.
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Th. Schalkhammer,E. Mann-Buxbaum,I. Moser,G. Hawa,G. Urban,F. Pittnerorders and infectious diseases along with cancer diseases. For instance, in case of pulmonary tuberculosis, hsa_circRNA_001937 was upregulated, while hsa_circRNA_102101 got downregulated; Hsa_circ_000178 was depicted to get upregulated in breast cancer which is associated with disease progression. F
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