Titlebook: Immersive Systemic Knowing; Advancing Systems Th Raghav Rajagopalan Book 2020 Springer Nature Switzerland AG 2020 Immersive Systemic Knowin

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Immersive Systemic Knowing978-3-030-49135-2Series ISSN 1568-2846

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Raghav Rajagopalans and dose rates, however, still remain ambiguous (Pohl-Rüling 1990; Sagan 1987). The study of interaction of low dose irradiation with subsequent mutagenic treatment could be a way of evaluating the effects of low doses of irradiation. This type of research has linked to a general biological phenom

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Raghav Rajagopalan, 2007), collectively referred to as pluripotent stem cells (hPSCs), are currently used in disease modeling to address questions specific to humans and to complement our insight gained from model organisms (Soldner et al. Cell 146:318–331, 2011; Soldner and Jaenisch, Science 338:1155–1156, 2012). Re

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Raghav Rajagopalan retroelement of specific chromatin states or DNA-bound protein complexes. A well-studied example of such a targeted retroelement is the . Ty5 retrotransposon, which integrates into heterochromatin at the telomeres and silent mating loci. Targeting is mediated by an interaction between Ty5 integrase

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Raghav Rajagopalanbination in ., these protocols have reduced dependence on restriction endonucleases, allowing the fabrication of complex TV constructs with relative ease. Using a methodology based on phage-plasmid recombination, we have developed a comprehensive TV construction protocol dubbed Orpheus recombination