Titlebook: Imaging and Quantifying Neuronal Autophagy; Ben Loos,Esther Wong Book 2022 Springer Science+Business Media, LLC, part of Springer Nature 2

拥护

并置

Ultrastructure of the Macroautophagy Pathway in Mammalian Cells, and quantify the amount of autophagic structures in cells and tissues. However, identification of autophagosomes, amphisomes, and autolysosomes in transmission electron microscopy sections requires experience and caution. The purpose of this chapter is to provide guidelines for correct identificati

endoscopy

Live Imaging of Autophagosome Biogenesis and Maturation in Primary Neurons, biogenesis occurs in the distal axon; newly formed autophagosomes engulf cargos including mitochondrial fragments, protein aggregates, and bulk cytoplasm. Once formed, autophagosomes move rapidly and processively along the axon toward the soma, fusing with lysosomes in transit to mature into degrad

Engaging

,Monitoring Autophagic Activity In Vitro and In Vivo Using the GFP-LC3-RFP-LC3ΔG Probe,l to elucidate the roles and mechanisms of autophagy. Although several relevant methods have been reported, the quantitative monitoring of autophagic activity in cells and in animals remains challenging. This chapter provides methods involving the recently developed autophagic flux probe GFP-LC3-RFP

补角

连锁,连串

Measuring Autophagosome Flux,g, of portions of the cytoplasm through autophagy eliminates unused and toxic proteins and organelles, promoting a functional proteome and cellular function. Autophagy also serves as an important adaptive mechanism that protects against metabolic perturbation. Loss of autophagy activity has major de

pantomime

Measurement of Neuronal Tau Clearance In Vivo, of zebrafish allows visualization of fluorescently tagged proteins within cells and the evaluation of changes in protein levels. Here, we describe a fluorescent-based technique using the photoconvertible protein Dendra fused to tau to analyze the clearance rate of Dendra-tau protein in neurons in t

深渊

Methods for Studying Axonal Autophagosome Dynamics in Adult Dorsal Root Ganglion Neurons, mature acidic lysosomes are relatively enriched in the soma. Long-distance transport of autophagosomes generated at distal axons is a key step for efficient degradation of autophagic vacuoles (AVs) via lysosomes in the soma. However, the mechanisms underlying the regulation of axonal autophagosome

leniency

Imaging and Quantifying Neuronal Autophagy to Determine the Autophagy Contribution to Neuronal and as well as recycling the building blocks. Autophagy inactivation has been shown to cause neurodegeneration, and autophagy dysfunction is linked with neurodegenerative diseases. Furthermore, accumulating evidence indicates that autophagy is involved in synaptic biology and plasticity, and the dysfunc

irreparable