Titlebook: Imaging Cellular and Molecular Biological Functions; Spencer L. Shorte,Friedrich Frischknecht Book 2007 Springer-Verlag Berlin Heidelberg

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Design and Function of a Light-Microscopy Facilitysearch infrastructure, in the form of services and facilities, is therefore an increasingly important foundation for a competitive research institution. A lightmicroscopy facility is a place of dynamic interaction among users, staff, and equipment. Staff provide the organization, continuity, and exp

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Quantitative Colocalisation Imaging: Concepts, Measurements, and Pitfallsinteraction. However, the incomplete separation of the different colour channels due to the presence of autofluorescence, along with cross-excitation and emission ‘bleed-through’ of one colour channel into the other, all combine to render the interpretation of multiband images ambiguous. Traditional

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Quantitative FRET Microscopy of Live Cellsng cells. FRET microscopy is increasingly employed to access the molecular mechanisms governing diverse cellular processes such as vesicular transport, signal transduction and the regulation of gene expression. However, evaluation of experimental approaches for FRET microscopy and the data they prod

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Fluorescence Photobleaching and Fluorescence Correlation Spectroscopy: Two Complementary Technologiest widely used techniques employed to study the transport and diffusion as well as the interaction and immobilisation of biological molecules inside the cellular environment. This has been promoted by the emergence of fluorescent proteins for in vivo labelling and the development of confocal laser s

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From Live-Cell Microscopy to Molecular Mechanisms: Deciphering the Functions of Kinetochore Proteinsscopy has emerged as the method of choice for probing molecular function in a nearphysiological environment. However, light-microscopy data are on the cellular scale, while data interpretation occurs on the molecular scale. To bridge the gap between these two scales, empirical mathematical models of

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Culmination

Imaging Parasites in Vivoin the development of molecular tools and imaging techniques have allowed to gain exciting new insights into these interactions on the cellular and molecular level. Often these studies have led to the discoveries of basic cellular mechanisms that the pathogen, be it virus, bacterium, fungus or paras

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