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Titlebook: IT-Betrieb; Management und Betri Bernd Pfitzinger,Thomas Jestädt Book 2016 The Editor(s) (if applicable) and The Author(s) 2016 BYOD.Cloud.

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.-acting factor (antibody supershift assay), is used to identify proteins present in the DNA-protein complex. The gel-shift competition assay with an unlabeled probe containing a slightly different sequence is a powerful technique used to assess the sequence specificity and relative binding affinit
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Bernd Pfitzinger,Thomas Jestädteir cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome
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Bernd Pfitzinger,Thomas Jestädt that control gene expression under different physiological stresses have been further characterized in the context of heterologous promoters to demonstrate their “stand-alone” functional properties in the absence of confounding influences from other .-elements and their cognate transcription factor
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Bernd Pfitzinger,Thomas Jestädtmethod. In this chapter, we introduce how to design and generate miRNA tough decoy inhibitors with an adeno-associated viral construct. TuD inhibitors will have two miRNA binding sites. The TuD will include stem sequences, a miRNA binding site, and linkers. In vitro validation experiments to confirm
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