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Titlebook: Hypertension; Methods and Protocol Jérôme P. Fennell,Andrew H. Baker Book 2005 Humana Press 2005 blood pressure.cardiovascular.gene therapy

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Congenic/Consomic Models of Hypertensionble for blood-pressure regulation by genome-wide scanning. In this chapter we focus on congenic and consomic breeding strategies for the confirmation of QTL and the genetic dissection of the implicated regions.
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Extraction of RNA From Cells and Tissueom homogenized tissues or cultured cells using such reagents, together with additional purification steps by passage through Phase-Lock gel tubes, and recommend optional chromatographic poly(A+) RNA enrichment procedure using Mini Oligo(dT) spin columns, together with the spectrophotometric determination of RNA yield and purity.
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TaqMan Real-Time PCR Quantification Conventional and Modified Methods, we were able to measure very small amounts of DNA template, and succeeded in comparing the levels of aldosterone synthase gene expression in samples of cardiac tissue from patients diagnosed with heart failure and control subjects.
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