| 书目名称 | Hydrocarbon and Lipid Microbiology Protocols | | 副标题 | Synthetic and System | | 编辑 | Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale | | 视频video | http://file.papertrans.cn/431/430330/430330.mp4 | | 概述 | Offers readily-reproducible step-by-step laboratory methods.Provides helpful tips and tricks supporting the protocols.Includes troubleshooting advice.Contains practical laboratory guidance to promote | | 丛书名称 | Springer Protocols Handbooks | | 图书封面 |  | | 描述 | This Volume presents generic protocols for wet experimental and computer-based systems and synthetic biology approaches relevant to the field of hydrocarbon and lipid microbiology. It complements a second Volume that describes protocols for systems and synthetic biology applications. The wet experimental tools presented in this Volume include protocols for the standardisation of transcriptional measurements, application of uracil excision-based DNA editing for, inter alia, multi-gene assembly, the use of fluxomics to optimise “reducing power availability”, and the incorporation of non-canonical amino acids into proteins for optimisation of activities. Phenome-ing microbes, using a combination of RNA-seq and bioinformatic algorithms, is presented, as is an illustration, using methylotrophs as an example, of how the different key omics approaches constitute a pipeline for functional analysis, acquisition of a systems overview, and metabolic optimisation. Complementary computational tools that are presented include protocols for probing the genome architecture of regulatory networks, genome-scale metabolic reconstruction, and bioinformatic approaches to guide metabolic engineering. Th | | 出版日期 | Book 2016 | | 关键词 | Environmental transcription factors; Expression systems; Fluxomics and metabolics; Higher-structure gen | | 版次 | 1 | | doi | https://doi.org/10.1007/978-3-662-50432-1 | | isbn_softcover | 978-3-662-57058-6 | | isbn_ebook | 978-3-662-50432-1Series ISSN 1949-2448 Series E-ISSN 1949-2456 | | issn_series | 1949-2448 | | copyright | Springer-Verlag Berlin Heidelberg 2016 |
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