| 书目名称 | Hydrocarbon and Lipid Microbiology Protocols |
| 副标题 | Single-Cell and Sing |
| 编辑 | Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale |
| 视频video | |
| 概述 | Offers readily reproducible, step-by-step laboratory methods.Provides helpful tips and tricks to complement the protocols.Includes troubleshooting advice.Features practical laboratory guidelines to pr |
| 丛书名称 | Springer Protocols Handbooks |
| 图书封面 |  |
| 描述 | This Volume presents relevant single-cell and single-molecule approaches in the study of microbes producing and utilizing hydrocarbons and lipids. While generically applicable for all microorganisms, the approaches described are, wherever possible, adapted to the field of study of hydrocarbon and lipid microbiology. The methods include basic procedures for isolating single cells by means of microfluidics and flow cytometry, and their cultivation in arrays as pure clones; for isolating, amplifying and sequencing single-cell genomes and transcriptomes; and for analysing single-cell metabolomes by means of Raman spectroscopy. Single-molecule approaches include the use of protein:fluorescent dye fusions for protein localization and methods for the production of cell division protostructures and lipid monolayers. Methods for the functional analysis of single cells include detection of metabolically active (protein-synthesizing) cells in environmental samples by bioorthogonal non-canonical amino acid tagging, Raman spectroscopy combined with stable isotope labelling and fluorescent in situ hybridisation, and visualization of single cells participating in gene transfer activity. Lastly, p |
| 出版日期 | Book 2016 |
| 关键词 | Bioreporters; Functional flow cytometry; Metabolomics; Protein localisation with GFP fusions; Proteomics |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-3-662-49131-7 |
| isbn_softcover | 978-3-662-56982-5 |
| isbn_ebook | 978-3-662-49131-7Series ISSN 1949-2448 Series E-ISSN 1949-2456 |
| issn_series | 1949-2448 |
| copyright | Springer-Verlag Berlin Heidelberg 2016 |
发表于 2025-3-21 19:43:11
