书目名称 | Hydrocarbon and Lipid Microbiology Protocols |
副标题 | Biochemical Methods |
编辑 | Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale |
视频video | |
概述 | Offers readily-reproducible step-by-step laboratory methods.Provides helpful tips and tricks supporting the protocols.Includes troubleshooting advice.Contains practical laboratory guidance to promote |
丛书名称 | Springer Protocols Handbooks |
图书封面 |  |
描述 | This Volume provides protocols for the biochemical analysis of hydrocarbon- and lipid-relevant products, cell components and activities of microbes that interact with hydrophobic compounds. They include methods for the extraction, purification and characterisation of surface tension-reducing bioemulsifiers and biosurfactants that increase the surface area and hence bioavailability of hydrophobic substrates. Protocols for the isolation and biochemical analysis of lipids and polyhydroxyalkanoates, food storage products made during nutrient abundance that represent important biotechnological products, are presented. The extraction of membrane lipid rafts, sub-organelles that fulfil important functional roles for the cell membrane, and the isolation and characterisation of membrane phospholipid biomarkers, are also described. The purification and characterisation of integral membrane hydrocarbon-oxidising enzymes are addressed. Lastly, two generic methods for the genetic analysis of catabolic pathways and analysis of ligand binding are presented..Hydrocarbon and Lipid Microbiology Protocols.There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and |
出版日期 | Book 2016 |
关键词 | Biomarkers; Hydrocarbon Biodegradation; Hydrocarbon Transport Proteins; Lipid Analysis; Lipopeptide Anal |
版次 | 1 |
doi | https://doi.org/10.1007/978-3-662-49137-9 |
isbn_softcover | 978-3-662-56984-9 |
isbn_ebook | 978-3-662-49137-9Series ISSN 1949-2448 Series E-ISSN 1949-2456 |
issn_series | 1949-2448 |
copyright | Springer-Verlag Berlin Heidelberg 2016 |