Titlebook: Hydrocarbon and Lipid Microbiology Protocols; Activities and Pheno Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale Book 2017 Springer-Ve

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Measuring the Impact of Hydrocarbons on Rates of Nitrogen Fixation,n content, crude oil contamination through natural seepage or anthropogenic activities leads to an overload of the affected environment with organic carbon. Degradation of oil hydrocarbons by microorganisms is therefore limited by the availability of fixed nitrogen. Studies of bioremediation of crud

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,Bacterial Solvent Responses and Tolerance: ,–, Isomerization, of .- to .-unsaturated membrane fatty acids as a tool for the measurement of the toxicity of membrane-disturbing compounds. The degree of isomerization directly depends on the toxicity and concentration of membrane-affecting agents. Synthesis of . fatty acids is apparent within 30 min after additio

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Protocols for Measuring Biosurfactant Production in Microbial Cultures,e these amphiphilic molecules. Literature on the production and analytical detection of biosurfactants is overwhelmed with assertions of high yields for such products which are mostly over exaggerated estimates due to the use of flawed or inaccurate analytical techniques. In this chapter we focus on

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Analysis of PHB Metabolism Applying Tn, Mutagenesis in ,, transposons “jump” within the genome, molecular biology uses plasmid-bound transposons, which randomly disrupt genomic regions of the target organism. Obtained transposon mutants help to elucidate metabolic pathways and to identify essential genes, which are involved in syntheses or degradation of

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Phenotyping Microarrays for the Characterization of Environmental Microorganisms,hich each well represents a different selective growth environment are important tools (1) in the identification of microbial isolates, (2) in the characterization of the phenotypic fingerprint of microbial communities, (3) for linking specific functions with specific organisms or genes, and (4) for

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Protocol for Evaluating the Biological Stability of Fuel Formulations and Their Relationship to Care. This phenomenon is particularly acute in anaerobic sulfate-rich environments. It is therefore important to reliably assess the inherent susceptibility of fuels to anaerobic biodegradation in marine waters as well as provide a measure of the impact of this metabolism on the integrity of steel. Suc

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Protocols for Measuring Methanogenesis,nt times, methane was known as a combustion gas, but its microbiological origin was proved only in the nineteenth century. The contribution of methane to the global warming and its beneficial importance in ecological biotechnology and bioenergetics dictate the need in proper estimations of its fluxe