| 书目名称 | Human Retrovirus Protocols | | 副标题 | Virology and Molecul | | 编辑 | Tuofu Zhu | | 视频video | | | 概述 | Includes supplementary material: | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection. | | 出版日期 | Book 2005 | | 关键词 | DNA; Microarray; PCR; RNA; Termination; gene expression; genes; molecular biology; reproducible techniques; v | | 版次 | 1 | | doi | https://doi.org/10.1385/1592599079 | | isbn_softcover | 978-1-61737-599-6 | | isbn_ebook | 978-1-59259-907-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2005 |
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发表于 2025-3-21 20:03:42
