| 书目名称 | Human Pluripotent Stem Cells | | 副标题 | Methods and Protocol | | 编辑 | Philip H. Schwartz,Robin L. Wesselschmidt | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments. Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better. in vitro. human disease models; 2) the discovery that a Rho-associated protein Kinase (ROCK) inhibitor allows for efficient single cell passaging and cryopreservation, increasing the efficiency and reliability of hPSC culture; and 3) defined, animal-component-free media, which lay the groundwork for simplified scale-up for therapeutic applications, differentiation protocols, and toxicology screens. The aforementioned technologies can be found in .Human Pluripotent Stem Cells: Methods and Protocols., a compilation of 33 detailed protocols in six categories of PSC research that cover laboratory essentials and the derivation of new PSC lines, including induced PSC lines, as well as their growth, maintenance, charact | | 出版日期 | Book 2011 | | 关键词 | cardiomyocytes; dopamine neurons; episomal transgene expression; gene expression analysis; human fibrobl | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-61779-201-4 | | isbn_softcover | 978-1-4939-5802-3 | | isbn_ebook | 978-1-61779-201-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC 2011 |
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发表于 2025-3-21 16:32:24
