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Titlebook: Human Naïve Pluripotent Stem Cells; Peter Rugg-Gunn Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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发表于 2025-3-21 19:12:44 | 显示全部楼层 |阅读模式
书目名称Human Naïve Pluripotent Stem Cells
编辑Peter Rugg-Gunn
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Human Naïve Pluripotent Stem Cells;  Peter Rugg-Gunn Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to
描述.This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Cutting-edge and thorough, .Human Naïve Pluripotent Stem Cells: Methods and Protocols. is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field..
出版日期Book 2022
关键词reprogramming; naïve pluripotency; preimplantation embryos; transcriptional datasets; Crispr-Cas9
版次1
doihttps://doi.org/10.1007/978-1-0716-1908-7
isbn_softcover978-1-0716-1910-0
isbn_ebook978-1-0716-1908-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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,Generating Trophoblast Stem Cells from Human Naïve Pluripotent Stem Cells,s indispensable for a healthy pregnancy. Epithelial cells in the placenta, which are termed trophoblasts, originate from the trophectoderm (TE) compartment of the blastocyst. The human trophoblast lineage consists of several distinct cell types, including the self-renewing and bipotent cytotrophobla
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,Differentiation and Expansion of Human Extra-Embryonic Endoderm Cell Lines from Naïve Pluripotent Suring blastocyst development, and the second being gastrulation-stage definitive endoderm (DE). The PrE gives rise to the primary and secondary yolk sac, and has supportive functions during pregnancy for nutrient provision, with descendants of this extra-embryonic lineage also playing a role in embr
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,Capacitation of Human Naïve Pluripotent Stem Cells,ibes a simple experimental system for the efficient and consistent transition of human pluripotent stem cells (hPSCs) from the naïve to the primed state, which is a process called capacitation. Naïve hPSCs after capacitation can be differentiated further to somatic lineages, thus reproducing the ord
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