书目名称 | Human Naïve Pluripotent Stem Cells | 编辑 | Peter Rugg-Gunn | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Cutting-edge and thorough, .Human Naïve Pluripotent Stem Cells: Methods and Protocols. is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field.. | 出版日期 | Book 2022 | 关键词 | reprogramming; naïve pluripotency; preimplantation embryos; transcriptional datasets; Crispr-Cas9 | 版次 | 1 | doi | https://doi.org/10.1007/978-1-0716-1908-7 | isbn_softcover | 978-1-0716-1910-0 | isbn_ebook | 978-1-0716-1908-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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