书目名称 | Human Monoclonal Antibodies |
副标题 | Methods and Protocol |
编辑 | Michael Steinitz |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma; production and purification of human monoclonal antibodies; humanization and optimization of monoclonal antibodies; rapid chimerization of monoclonal antibodies; epitope mapping via phage display from single gene libraries; recombinant antibodies made by combining phage and yeast display selections; production of stabilized antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells; and analysis of CAR T cells. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Uniqueand thorough, .Human Monoclonal Antibodies: Methods and Protocols, Second Edition. is a valuable tool for novice and expert researchers interested in learning more about this evolving field.. |
出版日期 | Book 2019Latest edition |
关键词 | transgenic mice; phage display; Alzheimer’s disease; melanoma; T-cells; antibodies |
版次 | 2 |
doi | https://doi.org/10.1007/978-1-4939-8958-4 |
isbn_ebook | 978-1-4939-8958-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media, LLC, part of Springer Nature 2019 |