Titlebook: Human Embryonic Stem Cell Protocols; Kursad Turksen Book 2016Latest edition Springer Science+Business Media New York 2016 Derivation of ce

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Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR,ve quantification of target genes, including undifferentiation and differentiation markers when referred to a housekeeping control with the addition of a calibrator that serves as an internal control to compare different lots of reactions during the time. The main aspects will include a minimal numb

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Study of Gap Junctions in Human Embryonic Stem Cells,iological events. GJIC is required for mouse embryonic stem cell maintenance and proliferation and various studies suggest that functional GJIC is a common characteristic of human embryonic stem cells (hESC) maintained in different culture conditions. This chapter introduces methods to study gap jun

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Genetic Manipulation of Human Embryonic Stem Cells, genetic modifications while remaining pluripotent, and can be selectively propagated, allowing the clonal expansion of genetically altered cells in culture. Since the first isolation of ES cells in mice, many effective techniques have been developed for gene delivery and manipulation of ES cells. T

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Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 Syced pluripotent stem cells (iPSCs) by defined factors (Takahashi et al., Cell 131:861–872, 2007) provides a renewable source of patient autologous cells that not only retain identical genetic information but also give rise to many cell types of the body including neurons and glia. Meanwhile, the rap

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Use of Multicolor Flow Cytometry for Isolation of Specific Cell Populations Deriving from Differentive cells. Here we describe a multicolor flow cytometry technique that uses five distinct cell surface antigens to isolate four live populations with different surface antigen profiles. These profiles were used to help distinguishing between neural and nonneural (the lens) ectoderm derivatives withi

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