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Titlebook: Human Embryonic Stem Cell Protocols; Kursad Turksen Book 20061st edition Humana Press 2006 DNA.biology.cancer.cell.microarray.mitochondria

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发表于 2025-3-21 18:09:45 | 显示全部楼层 |阅读模式
书目名称Human Embryonic Stem Cell Protocols
编辑Kursad Turksen
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Human Embryonic Stem Cell Protocols;  Kursad Turksen Book 20061st edition Humana Press 2006 DNA.biology.cancer.cell.microarray.mitochondria
描述A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
出版日期Book 20061st edition
关键词DNA; biology; cancer; cell; microarray; mitochondria; molecular biology; stem cell; stem cells; tissue; Cell c
版次1
doihttps://doi.org/10.1385/1597450464
isbn_ebook978-1-59745-046-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2006
The information of publication is updating

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发表于 2025-3-21 20:33:54 | 显示全部楼层
Manipulation of Self-Renewal in Human Embryonic Stem Cells Through a Novel Pharmacological GSK-3 Inating into a specific type or random heterogenous cell populations in vitro. These approaches will become a fundamental platform to identify molecular networks regulating cell fate determination in hESCs and mouse embryonic stem cells.
发表于 2025-3-22 03:46:01 | 显示全部楼层
Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryong factors influential on self-renewal of hESCs. Also described in this chapter: a protocol and tips for conducting gene chip analysis focusing on widely used Affymetrix microarrays. These techniques will provide an unprecedented scale of biological information to illuminate a key to decipher complex signaling networks controlling pluripotency.
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Culture of Human Embryonic Stem Cells on Human and Mouse Feeder Cells,by trypsin/ethylenediaminetetraacetic acid-mediated dissociation. We also describe methods we use to characterize hES cells expanded on either human or mouse feeder cells, including alkaline phosphatase staining, immunostaining for cell-surface markers associated with undifferentiated hES cells, and teratoma formation in mice.
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1064-3745 the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.978-1-59745-046-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
发表于 2025-3-23 00:27:44 | 显示全部楼层
In Vitro Differentiation of Neural Precursors From Human Embryonic Stem Cells,ation accounts for at least 70% of the total differentiated progenies. Thus, our system not only provides a source of synchronized neuroectodermal cells, but also offers a paradigm to dissect mechanisms of neural induction and cell lineage specification during early human development.
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