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派博传思国际中心 »期刊书目

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Titlebook: Human Embryonic Stem Cell Protocols; Kursad Turksen Book 2010Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi

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发表于 2025-3-21 19:17:30 | 显示全部楼层 |阅读模式
书目名称Human Embryonic Stem Cell Protocols
编辑Kursad Turksen
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概述Comprises a comprehensive guide to isolate culture and differentiate human embryonic stem cells in vitro.Provides easily followed detailed protocols.Covers numerous essential methods for culturing and
丛书名称Methods in Molecular Biology
图书封面Titlebook: Human Embryonic Stem Cell Protocols; Kursad Turksen Book 2010Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi
描述.Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, new freedoms in regard to using them for research has allowed interest to remain high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In .Human Embryonic Stem Cell Protocols, Second Edition., internationally respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. As a volume of the .Methods in Molecular Biology.™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls...Comprehensive and cutting-edge, .Human Embryonic Stem Cell Protocols, Second Edition. offers both novice an
出版日期Book 2010Latest edition
关键词Cell culture; HES cells; Lineage commitment; PCR; Proteomics; Regenerative medicine; Self-renewal; cell lin
版次2
doihttps://doi.org/10.1007/978-1-60761-369-5
isbn_softcover978-1-4939-6107-8
isbn_ebook978-1-60761-369-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Embryos,ether because the couple in question has completed their family or because the embryo was found to be clinically unsuitable for transfer due to severe genetic condition (in case of pre-implantation genetic diagnosis, PGD). Culturing embryos to a blastocyst stage (5–6 days after IVF) before embryo tr
Mechanics
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Allergic
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Serum-Free and Feeder-Free Culture Expansion of Human Embryonic Stem Cells,o studying human diseases and genetic functions of healthy organisms. The rate at which hESCs are being used in laboratories is exponentially increasing, and with that, the need for xeno-free hESCs is also increasing. Xeno-free grade hESCs, cells that have not come into contact with any animal-deriv
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发表于 2025-3-22 20:08:23 | 显示全部楼层
Single Cell Enzymatic Dissociation of Human Embryonic Stem Cells: A Straightforward, Robust, and Ste of the cells‘ unique potential. In contrast to mouse embryonic stem cells, hES cells usually have to be expanded by tedious mechanical microdissection or by enzymatic dissociation to cell clusters of a very narrow size range..It is essential to use a culture system that allows the robust and repro
Metamorphosis
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Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR,ve quantification of target genes, including undifferentiation and differentiation markers when referred to a housekeeping control with the addition of a calibrator that serves as an internal control to compare different lots of reactions during the time. The main aspects will include a minimal numb
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