Titlebook: Human Cytomegaloviruses; Methods and Protocol Andrew D. Yurochko,William E. Miller Book 2014 Springer New York 2014 cytomegalovirus.fibrobl

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Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Part the cultures for cytopathic effects characteristic of this virus. Initially, such clinical isolates are usually strictly cell associated, but continued propagation in cell culture increases the capacity of an HCMV isolate to release cell-free infectious progeny. Once cell-free infection is possible

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Use of Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegaloviru and tremendous permissiveness for infection allow the study of all facets of infection, an abbreviated list of which includes ligand/receptor interactions, activation of cell signaling responses, and dysregulation of the cell cycle and DNA repair processes. Another advantage to fibroblasts’ permiss

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The Use of Primary Human Cells (Fibroblasts, Monocytes, and Others) to Assess Human Cytomegalovirustion of other cell types, such as undifferentiated cells of the myeloid lineage, gives rise to nonpermissive infections. This has been used experimentally to model latent infection which is known to be established in the pluripotent CD34+ hematopoietic progenitor cell population resident in the bone

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Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation,ture. Culturing hematopoietic subpopulations while maintaining physiological relevance must be given utmost consideration. We describe a long-standing primary CD34. hematopoietic progenitor cell (HPCs) system as an experimental model to study human cytomegalovirus (HCMV) latency and reactivation. Ke

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,Use of 5-Ethynyl-2′-Deoxyuridine Labelling and Flow Cytometry to Study Cell Cycle-Dependent Regulatation in the design and control of HCMV experiments. While G0/G1 cells support the immediate onset of viral transcription, cells progressing through the S and G2 cell cycle phases prevent HCMV from entering the lytic replication cycle. Here, we provide two fast and reliable protocols that allow one

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Methods to Study the Nucleocytoplasmic Transport of Macromolecules with Respect to Their Impact on egulation of gene expression. Accurate subcellular localization is crucial for the effective function of most viral macromolecules, and nuclear translocation is central to the function of herpesviral proteins that are involved in processes such as transcription or DNA replication. Human cytomegalovi