书目名称 | Human Cell Culture Protocols | 编辑 | Ragai R. Mitry,Robin D. Hughes | 视频video | | 概述 | Supplies techniques that can be adapted to obtain cell cultures from diseased cells to gain understanding of the pathogenic mechanisms involved.Features cutting-edge methods ready for the laboratory.I | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of .Human Cell Culture Protocols. expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the .in vitro. use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respectiv | 出版日期 | Book 2012Latest edition | 关键词 | Biocompatible scaffolds; Cell function; Diseased tissues; Hepatocytes; Human stem cells; Intracellular me | 版次 | 3 | doi | https://doi.org/10.1007/978-1-61779-367-7 | isbn_softcover | 978-1-4939-6208-2 | isbn_ebook | 978-1-61779-367-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC 2012 |
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