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Titlebook: Human Cell Culture Protocols; Gareth E. Jones Book 19961st edition Springer Science+Business Media New York 1996

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Growth and Differentiation of Human Adipose Stromal Cells in Culture,ved from subcutaneous or omental adipose tissue. Under specific culture condition, they will differentiate without replication into cells resembling mature adipocytes or will replicate, become confluent, and grow in subculture. The initial observation that the stromal vascular fraction of human omen
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Cell Culture of Human Brain Tumors on Extracellular Matrices,the purpose of karyotyping, chemoresistance testing, or basic research. It provides investigators with an opportunity to investigate many cellular parameters and interactions in an in vitro system in which the experimental conditions can be controlled and repeated With many tissues, either human or
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Human Thymic Epithelial Cell Cultures, the organ (several billions of thymocytes in a child’s thymus), but small numbers of other hematopoietic cells are found in the intrathymic microenvironment, such as thymic monocytes, macrophages, interdigitating dendritic cells, and B-cells Stromal cells of nonhematopoietic origm comprise thymic e
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Generation and Cloning of Antigen-Specific Human T-Cells,ll culture in vitro was the discovery of the T-cell growth-inducing properties of interleukin-2 (IL-2), originally called T-cell growth factor or TCGF, by Morgan et al (.), who for the first time were able to grow human T-lymphocytes, isolated from human bone marrow. Shortly thereafter, Gillis and S
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Purification of Peripheral Blood Natural Killer Cells,r lineages. NK cells are a discrete lymphocyte subset distinguishable from B- and T-lymphocytes on the basis of both physical and phenotypic characteristics that can be exploited for then purification. Techniques based on differential cell buoyancy (centrifugation on discontinuous density gradients,
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