Titlebook: Hox Genes; Methods and Protocol Yacine Graba,René Rezsohazy Book 2014 Springer Science+Business Media New York 2014 Biomedical applications

内向者

Chromatin Immunoprecipitation and Chromatin Immunoprecipitation with Massively Parallel Sequencing oh massively parallel sequencing (ChIP-seq) is a recently developed powerful tool to analyze genome-wide binding of transcription factors and histone modifications and provides a vast amount of information into the regulation of gene expression. This chapter describes how ChIP and ChIP-seq are performed on mouse embryonic tissue.

conformity

易改变

The Genetics of Murine , Loci: TAMERE, STRING, and PANTHERE to Engineer Chromosome Variantsn the structure of the gene cluster and the architecture of the surrounding genomic landscape is at play. Here, we report on a portfolio of in vivo genome engineering strategies in mice, which have been used to probe and decipher the genetic and molecular underpinnings of the complex regulatory mechanisms implemented at these loci.

运动性

Nebulous

ChIP for Hox Proteins from , Imaginal Discsa protocol for ChIP to identify binding of the Ultrabithorax (Ubx) Hox transcription factors from imaginal discs of . larvae. The protocol can be extended to other model organisms and transcription factors.

Mangle

Ferdinand Marlétaz,Jordi Paps,Ignacio Maeso,Peter W. H. Holland

营养

Delphine Paul,Caroline Sauvegarde,René Rezsohazy,Isabelle Donnay

AMPLE

水汽

骇人