Titlebook: Host-Pathogen Interactions; Methods and Protocol Steffen Rupp,Kai Sohn Book 2009 Humana Press 2009 Antigen.Bacterial pathogens.Fungal patho

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Surface-Exposed Adherence Molecules of ,actions. The specific interaction of bacterial proteins with host proteins often subverts the physiologic function of host-derived proteins, and therefore the bacterial proteins are considered as key players in the infectious process. The direct binding of host proteins is exploited by the pathogens

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Characterizing Host Receptor Recognition by Individual Bacterial Pathogenspecialized adhesins. The specific adhesin-receptor interaction allows pathogens to tightly bind to their target cells, thereby facilitating the colonization of host tissues. Therefore, the identification and characterization of bacterial adhesins is a major topic in infection biology. This chapter f

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A Method for Examining Glycans Surface Expression of Yeasts by Flow Cytometryell wall glycans play an important role in the continuous interchange that regulates the balance between saprophytism and parasitism and between resistance and infection. Flow cytometry is a useful method for probing surface yeast glycans in order to compare their expression depending on strains and

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Human Epithelial Model Systems for the Study of , Infections ,: Part I. Adhesion to Epithelial Modeld host, this process is crucial for colonization and subsequent penetration of the respective tissue. . analyses of the adhesion process in whole organisms are limited because of difficulties in providing reproducible and comparable conditions in the host environment. Therefore, . assays provide the

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Human Epithelial Model Systems for the Study of , infections ,: Part II. Histologic Methods for Studhe tools that exist to fill these gaps in knowledge, . tissue models based on reconstituted human epithelia (RHE) have already been developed. Such models are designed to study more reproducably the fungus-host relationship, as they eliminate the complexity and variability found .. Herein we describ

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Systems for Studying the Interaction of Fungal Pathogens with Primary Cells from the Mammalian Inna cohorts (e.g., HIV-infected patients, transplant recipients, immunosuppressed patients with cancer). Opportunistic fungal pathogens such as . spp. are most often associated with serious systemic infections. Currently available antifungal drugs are rather unspecific, often with severe side effects.