Titlebook: Host-Fungus Interactions; Methods and Protocol Alexandra C. Brand,Donna M. MacCallum Book 2012 Springer Science+Business Media, LLC 2012 Fu

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Gene Knockdown in , Using Antisense RNAsms underlying these phenotypes remain to be clarified, mostly due to the absence of efficient classical genetic and molecular techniques. Here we describe a method for gene expression knockdown in . by antisense RNA (aRNA) technology taking advantage of an .-mediated transformation (ATMT) system. T

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Tetracycline-Inducible Gene Expression in ,able promoters are available that enable researchers to shut off or turn on the expression of a target gene in ., usually by growing the cells in inducing or repressing media. In this chapter, we describe a tetracycline-inducible gene expression system (Tet-On) that allows forced expression of endog

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Galactose-Inducible Promoters in , var. ,g gene essentiality in .. Galactose-inducible promoters have been successfully used in . to manipulate heterologous gene expression. Utilizing . galactose-inducible genes in a BLAST search of the sequenced . var. . genome, we found three potential galactose-inducible promoters, P., P., and P. that a

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1064-3745 d reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. .Comprehensive and practical, .Host-Fungus Interactions: Methods and Protocols. des978-1-4939-5904-4978-1-61779-539-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

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nd ended up being destined mostly to the colonial market. Hence, the serious crisis experienced by the sector since the late-eighteenth century due to the interruption of trade with America as a result of the revolutionary wars and the subsequent emancipation of those territories. But the drastic re

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