Titlebook: Host-Bacteria Interactions; Methods and Protocol Annette C. Vergunst,David O‘Callaghan Book 2014 Springer Science+Business Media New York 2

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Detection of the Interaction Between Host and Bacterial Proteins: Eukaryotic Nucleolin Interacts wit therapeutic approaches, either preventive or curative. Here, we describe different techniques, which allowed us to detect new molecules involved in the binding and infection of the bacterium ., on human cells. This facultative intracellular pathogen is the causative agent of tularemia and is consid

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Hijacking the Host Proteasome for the Temporal Degradation of Bacterial Effectorsrious “effector proteins” which are delivered from the bacteria to the host cell cytosol. In order to orchestrate host cell reprogramming, the function of effectors inside host cells is regulated both temporally and spatially. In eukaryotes one of the most prominent processes used to degrade protein

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Investigating TLR Signaling Responses in Murine Dendritic Cells Upon Bacterial Infectionr by dendritic cells. Toll-like receptors (TLRs) are key in the detection of a variety of microbial stimuli..Here we focus on the methodology used to evaluate the role of TLRs in the process of dendritic cell response to bacterial intracellular infections, using bone marrow-derived dendritic cells (

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RNA Sequencing of FACS-Sorted Immune Cell Populations from Zebrafish Infection Models to Identify Cely life stages, is especially attractive for live imaging of interactions between pathogens and host cells. A number of useful fluorescent reporter lines have recently been developed and significant advances in RNA sequencing technology have been made, which now make it possible to apply the zebrafi

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