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Titlebook: Hormonally Defined Media; A Tool in Cell Biolo G. Fischer,R. J. Wieser Conference proceedings 1983 Springer-Verlag Berlin Heidelberg 1983 F

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3T3 Cell Growth Initiation and Multiplication in Defined Medium Supplemented with Growth Factors andfactors present in serum support 3T3 cell prolife-ration (1,2). Using swiss 3T3 mouse cells cultured in a suboptimal culture medium (DME) this laboratory has been involved for several years in characterizing serum growth factors in attempts to use them to substitute for serum in order to grow 3T3 ce
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Growth-Promoting Activity of Thrombin in Fibroblasts: Temporal Action and Early Biochemical Events iwever, the molecular mechanisms by which these extracellular signals act on quiescent cells to induce the G0→G1 transition, DNA replication and subsequent cell division or differentiation are unknown. The introduction by Sato and coworkers of cell culture in serum-free hormone-supplemented medium (1
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Pure Platelet-Derived Growth Factor Stimulates Human Fibroblasts to Proliferate in Plasma-Free Cultuo (2–5) and has been ascribed a role in wound healing (6) and in the pathogenesis of atherosclerosis (7). Data concerning the role of PDGF in the cell cycle are conflicting. For 3T3 cell cultures PDGF alone is not capable of inducing DNA synthesis but induces a state of “competence” to respond to ot
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The Secretion of Growth Regulatory Molecules by PC13 Embryonal Carcinoma Cellsced, by treatment with retinoic acid (RA), to undergo differentiation . . into a benign cell type, PC13 END, which phenotypically resembles the visceral endoderm of the early postimplantation mouse embryo (1,2). There is a pronounced change in growth regulatory properties associated with this differ
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Synergistic Interactions of Specific Prostaglandins in Regulating the Rate of Initiation of DNA Synte to physiological and pathological changes, as e.g. inflammation or cancer. The function of a prostaglandin depends on the target cell and the local presence of other prostaglandins and hormones (1, 2).
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