| 书目名称 | Homologous Recombination | | 副标题 | Methods and Protocol | | 编辑 | Andrés Aguilera,Aura Carreira | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This volume explores homologous recombination’s (HR) essential role in meiotic and somatic cells. It discusses the analysis of different steps of the HR process from the genetic, molecular biology, and cell biology perspectives. Using a variety of model systems, chapters in this book cover topics such as the genome-wide mapping of DNA double-strand breaks (DSB); analysis of DNA-end resection and recombination intermediates by gel electrophoresis and southern blotting; cell-based monitoring of HR activity in meiotic and mitotic cells; in vitro analysis of branch migration, DNA strand exchange and Holliday junction resolution; or super resolution imaging of HR repair at collapsed replication forks. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Homologous Recombination: Methods and Protocols. is a valuable resource that uses both classical and more recent approaches to answer questions on the HR mechanism. I | | 出版日期 | Book 2021 | | 关键词 | DNA breaks; recombination; Anaphase Bridges; HR repair; C; elegans | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-0716-0644-5 | | isbn_softcover | 978-1-0716-0646-9 | | isbn_ebook | 978-1-0716-0644-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2021 |
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发表于 2025-3-21 17:08:59
